Recombinant Protein Expression

Fusion Antibodies offers our high throughput FET™ platform service to rapidly develop reagents necessary for your research.  We combine our bio-informatic and proteomic expertise to produce high quality recombinant proteins.

The recombinant protein expression service is a ISO 9001:2000 certified service delivering high quality proteins that you can trust.


Advantages

Recombinant Proteins

  • Highly Bioactive
  • Successful expression of hard-to-produce proteins
  • High Solubility
  • Experts in refolding insoluble inclusion bodies
  • Rapid turnaround (~4 weeks)
Platforms

Track Record

  • Expressed proteins from 5 to 1150 amino acids
  • Purified over 500 recombinant proteins
  • Raised over 200 antibodies
  • More than 100 high quality clients

Our recombinant proteins can be used as bio-markers, diagnostic test proteins and also as excellent antigens, producing a strong immune response which yield antibodies in 99% of cases.

Examples Mammalian Targets – Cytokines, Receptors, Enzymes
Bacterial Targets – TB, Yersinia pestis
Viral Targets – Hepatitus B, Sars, Vaccinia

Accompanying Services

  • Polyclonal Antibody Production
  • Monoclonal Antibody Production
  • Antibody Sequencing
  • Antibody Engineering

Please enquire for more details on this service and pricing with our technical services team at technicalservices@fusionantibodies.com or call us on  +44(0) 28 9043 2800

Mammalian Recombinant Protein Expression

Fusion Antibodies ltd offers mammalian expression where the folding of the proteins is crucial for the requirements of the customer, and where the internal machinery in E.coli are unable to fully express normally large proteins of mammalian nature.
  • Stable and transient expression
  • Expression of large multimeric proteins
  • More complex post translational modifications
  • Production functionally active proteins
  • Disulphide bridges
  • Glycosylation

Transient expression services from world-leader Fusion Antibodies employ highly developed processes which are further customized to suit your specific requirements. Drug discovery or development, or high throughput screening; we have the extensive capabilities to meet a wide array of needs in parallel.

 

Technologically advanced, proprietary technology worked on by a team of experienced and committed scientists gives ‘rapid’ transient gene expression a new meaning. Our fast turnaround and reliable reagents enable our clients to move ahead with the clinical trials and drug development without having to wait it out through the lengthy stable transfection process.

 

In addition to our custom protein expression and purification services which are sought after by highly respected research institutions all over the world, our UK-based CRO offers a range of services producing reagents for all stages of therapeutic, bio similar and diagnostic antibody development. We’re your optimum partner in the development of your antibody target.

 

Contact us now to request a quote for bespoke transient expression services.

 

Cost-Effective Transient Expression Services

 

Why would you invest in stable cell line development if transient transfection is sufficient? It is an established process that is made even more efficient and effective by scientifically advanced technology like the one developed by Fusion Antibodies. We have combined our bioinformatics and proteomic expertise to produce high quality proteins. Our outstanding technical skills stem from our own drug discovery programme that has been utilized by drug development and diagnostic researchers around the world.

 

Transient gene expression allows you to

  • ·         Rapidly go from cloned gene to large quantities of protein in 3-7 days
  • ·         Obtain fully post-translationally modified and active mammalian proteins
  • ·         Use your available mammalian cell culture facilities and shake flasks and a platform shaker
  • ·         Easily purify secreted proteins from the serum-free cell culture medium
  • ·         Scale-up from 50 ml to 1 L of culture volume

 

Added to that is Fusion Antibodies’ ability to produce high quality, purified proteins from difficult to transfect cell lines at competitive rates.

 

If you’re looking to maximize time, yield and value, then bring us your requirements and we’ll draw up a detailed proposal with timelines and cost.

 

Call us on +44(0) 28 9043 2800 or send an email to inquire about competitively-priced transient expression services.