Selection of Host Species
hybridoma | b-cell cloning
Strategy, function and execution are the key to success. Once we’ve received your inquiry we’ll set up a call to get the detail that we need.
Fusion Antibodies will help select the host species that is best suited to generating the type of antibody you need.
Currently in development, OptiMAL® is Fusion’s new mammalian antibody library – a discovery platform yielding fully human antibodies.
hybridoma | b-cell cloning
20+ years expertise
State of the Art Support
Fusion Antibodies understand antibodies. Our experienced team will integrate our expertise with your understanding of the role you want your antibody to play. Whether a simple binder is needed for research assays, a highly specific diagnostic or a potential therapeutic candidate acting on complex biological systems, every project is different, every target is unique. Our long experience and expertise in generating antibodies has shown us the value of drawing from a repertoire of antigen design options, immunisation scheduling, host species and screening strategies to build successful antibody discovery projects.
Mouse and rat hybridoma technology, B-cell cloning strategies from rabbit and even diverse species such as chicken and more, each has its own characteristics and application. We will integrate antigen, immunisation and screening tools to exploit these traits. We can select the best approach in partnership with you to deliver an antibody that not only binds but one which works in the way you need it to.
When the Fusion Antibody Discovery Team take on a target we are starting from a position of having developed successful strategies for similar targets over the years. We have combined 17 years expertise and experience into generating a suite of immunogenic strategies specifically optimised to generate antibodies to a range of targets from small molecules to GPCRs and more.
Each strategy is selected for your project based on analysis of sequence, epitope availability, host-species immune response, as well as specificity requirements such as isoforms and PTMs and desired cross reactivity in the preclinical pathway.
This is carried out by experienced, dedicated scientists, interpreting output from a suite of state-of-the-art 3D protein structural modelling packages, epitope analysis and more; ensuring good alignment with the parental target and elimination of sequence liabilities.
For Fusion Antibodies, a properly designed screening strategy is the most vital component of a successful antibody discovery programme. We exploit every available opportunity to push the clones being selected, whether from a hybridoma or B-cell platform to find the antibodies that work.
Immunogen Design, Host-Species selection and screening are planned together, incorporating high-throughput screening with functional assays. Screening antigens are carefully designed and selected to complement the immunisation strategy, ensuring specificity in the clones selected. Target-Expressing stable CHO and HEK cell line pools can also be generated to confirm native protein activity.